With this technique, however, ethanol con sumption does not usually exceed 1020%. Natural products are one of the best ways to minimize ethanol hepatotoxicity. In ethanol experiments, ethanol 25% vv was administered by oral intubation5gkg body weight at the same time once a day control animals received an isocaloric dose of sucrose. Induction of cyp2e1 by ethanol appears to be one of the central pathways by which ethanol generates a state of oxidative stress. All animals had free access to standard rat chow ralston purina co. Hepatotoxicity was induced using 40% ethanol 1ml100gm b. Etoh, indicating that they are directly influenced by absolute etoh concentration.
Lieber alcohol research and treatment center and laboratory of liver disease and nutrition, bronx veterans administration medical center and mt. Jan 01, 2002 the results of the present investigation indicate that bile salts modulate hepatic disorders induced by chronic ethanol feeding in rats. Group iii and iv was given eept 500 and 750 mgkg, b. The present study was designed to evaluate the antioxidant activity of livomap on ethanol induced hepatotoxicity in albino wistar rats. Hepatoprotective activity of aqueous ethanol extract of ap was evaluated against repeated dose of diclofenac 150 mgkg, i. Liver damage was induced in rats by administering ethanol 5 gkg, 20% wv p. Rats fed ethanol from 21 to days were subjected to one or more episodes of hypoxia 6% o2 in order to determine if ethanol predisposed to centrilobular liver necrosis induced by hypoxia. Protective effects of garlic and silymarin on ndeainduced. However, mice receiving fc prior to ethanol treatment did not display hepatotoxicity as. Ethanol markedly elevated levels of serum aspartate aminotransferase ast and alanine aminotransferase alt in mice. Macroscopic view of ethanol induced longitudinal hemorrhagic bands arrow in glandular part of rat stomach a. Group ii, iii, iv, v were administered with 40% ethanol 1 ml100gm. Ethanol 6 gkg body weight bw and gte 200 mgkg bw were treated by gavage.
The rats were then observed for 48hours for signs of toxicity and death. The hepatoprotective activity of conyza bonariensis ethanol extract was studied against paracetamol induced hepatotoxicity in mice. Pdf ethanol induced hepatotoxicity in albino rats researchgate. Journal of the college of physicians and surgeons pakistan 2011, vol. Protective role of ficus benghalensis bark extract against. Autophagy could mitigate ethanolinduced hepatotoxicity by removing damaged mitochondria and accumulated fatty acids ethanolinduced cell death in vivo is minor, resulting in a modest increase in blood alt in most models of acute or chronic treatment without obvious gross histological changes unless other susceptibility factors exist figs. Abstract the present study was carried out to determine the severe hepatotoxicity and nephrotoxicity of gasoline on liver, kidney, hematological and oxidative stress parameters in wistar male albino rats.
Antioxidant effect of hemidesmus indicus on ethanol. Autophagy could mitigate ethanol induced hepatotoxicity by removing damaged mitochondria and accumulated fatty acids ethanol induced cell death in vivo is minor, resulting in a modest increase in blood alt in most models of acute or chronic treatment without obvious gross histological changes unless other susceptibility factors exist figs. Indira please note, due to essential maintenance online purchasing will be unavailable between 08. Effects of curcumin on cadmiuminduced hepatotoxicity in rats. The results of present work indicate that ajme has a significant protective effect against ethanol induced hepatotoxicity in chicks, which may be due to their antioxidant potential. Hepatoprotective effect of ethanolic extract of trichosanthes. Hepatotoxicity was induced in wistar male rats by oral administration, 2 gkg body weight on 7th day after the administration of ethanolic. Increased hepatotoxicity of acetaminophen after chronic ethanol consumption in the rat chifumi sato, yoshiro matsuda, and charles s. This indicates the prevention of hepatotoxicity related fatty changes in liver and improvement in liver function which leads. Ameliorative effect of flaxseed linum usitatissimum and. Hepatoprotective activity of methanol extract of tecomella undulata against alcohol and paracetamol induced hepatotoxicity in rats d singh, rs gupta reproductive physiology section, centre for advanced studies, department of zoology, university of rajasthan, jaipur 302055, india. The rats were divided into eight groups of which two served as control group i. Methods animal preparation the experimental preparation used in this study is. This research was to investigate several guava leaf extracts hepatoxic effect on healthy clone 9 liver cells.
Prevention of ethanolinduced hepatotoxicity by fermented. We investigated the effects and the possible mechanisms of silymarin on liver injury induced by acute ethanol etoh administration. Fc was investigated in male c57bl6 mice under ethanol induced oxidative stress. Pretreatment of the rats with ethanolic extract before pcm administration caused a significant reduction in the values of sgot, sgpt, alp, total and direct bilirubin, and triglycerides. Pdf twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each.
The antioxidant effect of the ethanolic root extract of hemidesmus indicus, an indigenous ayurvedic medicinal plant used in soft drinks in india, was studied in rats with ethanol induced hepatotoxicity. Antioxidant activity of graptopetalum paraguayense e. Effects of chronic ethanol consumption and one day food deprivation on the hepatotoxicity of low dose carbon tetrachloride ccl4. Microscopic view of ethanol induced mucosal lesion limited area and submucosal edema, 40 a. Effect of hesperidin and ascorbic acid on serum total protein, albumin and bilirubin in ethanol induced hepatotoxic rats 94 4. This study was performed to investigate the effects of green tea extracts gte on acute ethanol induced hepatotoxicity in rats. The results of the present study of the use of camel urine against alcohol hepatotoxicity in rats indicated a significant hepatoprotective effect. Autophagy reduces acute ethanolinduced hepatotoxicity and. In the absence of reliable liver protective drugs, a large number of natural medicinal preparations are used for the treatment of liver diseases. In prophylactic in vivo studies with the ethanol induced hepatotoxicity in rats rats subject category. The animals were treated once daily by oral gavage for five days and divided into four groups. Sp was administered to hepg2 human hepatocytes in the presence of ethanol, and also intravenously injected into mice with alcoholic liver damage.
The characterization of bo seed extract was performed using standard biochemical analysis. Effect of oleogumresin on ethanolinduced hepatotoxicity in. Dgalactosaminelipopolysaccharideinduced hepatotoxicity. Original article antioxidant and hepatoprotective effect of. Original article antioxidant and hepatoprotective effect. This study aims to investigate possible hepatoprotective activities of ethanolic extract of trichosanthes lobata against paracetamol induced hepatotoxicity. Protective effects of commelina benghalensis linn root extract on ethanol induced acute hepatotoxicity in rats sambrekar 1sudhir n, patil suhas a2 dept of pharmacology, maratha mandals college of pharmacy, belgaum, india, dept of pharmacognosy, maratha mandals college of pharmacy, belgaum, india.
In vitro antioxidant activity determined using dpph. Dept of pharmacology, maratha mandals college of pharmacy, belgaum, india, dept of pharmacognosy, maratha mandals college of pharmacy, belgaum, india. Histopathological and histochemical assessment of the protective. Hepatoprotective effect of urine of onehumped camel. Hepatoprotective potential of andrographis paniculata aqueous. Pdf the effect of ethanol extract mymercodia pendans on. The next day, they were presented again with n1 and also a bead from a new rat scagen2. Effect of hesperidin and ascorbic acid on liver marker enzymes in serum of ethanol induced hepatotoxic rats 96 5. Minneapolis veterans affairs medical center, minneapolis, minnesota 5541 7, virginia piper cancer institute of. Decarti aliuhvl rtwardi and treatment center, section of liver disease and nutrition, bronx va medical centerand mount sinai school o medicine, new york, ny, united slates of america historical perspective the association between. After an overnight fast, rats were given ethanol 5 gkg, 30% wtvol. Hepatoprotective activity of ethanol extracts of ficus. Liver protective activity of indoneesiella echioides. Mechanisms of hepatotoxicity toxicological sciences.
Kuntze extract ameliorates chronic ethanolinduced hepatotoxicity in albino rats. The results of the present investigation indicate that bile salts modulate hepatic disorders induced by chronic ethanol feeding in rats. The rats were divided into 3 groups n6 and administered 500, and 1500mgkg body weight ethanol extract of ficus exasperata leaf orally. Protective effect and antioxidant role of sweetgum liquidambar orientalis oil against carbon tetrachloride induced hepatotoxicity and oxidative stress in rats. The reasons for combining camel milk and urine together are. Figure 3 depicts a working hypothesis of the role of cyp2e1 in ethanol induced oxidative stress and hepatotoxicity. In our study, gastric mucosal injury was induced by the intragastric administration of 1 ml of absolute ethanol. Hepatotoxicity from hepatic toxicity implies chemicaldriven liver damage.
The activity of extract was also comparable to that of silymarin, a standard hepatoprotective drug. Effect of methanol extract of gomphrena celesioides on. The liver is the major target organ of ethanol toxicity 2. Aqueous extract of vernonia amygdalina protects against alcoholinduced hepatotoxicity in wistar rats. The protective effects of aqueous extracts of the fruit rind of garcinia indica gie on ethanol induced hepatotoxicity and the probable mechanisms involved in this protection were investigated in rats. To investigate the protective effect of the leaf extract of andrographis paniculata ap against ethanol induced liver toxicity in male albino rats. The present study was carried out to evaluate the hepatoprotective effect and antioxidant role of grape vitis vinifera l. Jun 15, 2018 to examine the effect of sp on ethanol induced hepatotoxicity, we used in vitro 30,31 and in vivo 32,33 models of ethanol induced liver damage. The effects of these treatments were evaluated by biochemical and western blot studies. Administering 20% ethanol 5 gkg of body weightday for 60 days to male wistar rats resulted in significantly decreased body weight and increased liverbody weight ratio. Effects of portulaca oleracea extract on acute alcoholic liver injury. Hepatoprotective effect of garcinol in acute paracetamol. The role of tumor necrosis factorcx in acute endotoxin induced hepatotoxicity in ethanol fed rats john hansen, david l. Green tea extract ameliorates ethanol induced liver injury in.
Protective effect of hericium erinaceus on alcohol induced. This study was undertaken to evaluate the effects of chronic ethanol consumption on the hepatotoxicity of acetaminophen. Silymarin protects against acute ethanolinduced hepatotoxicity in. A total of 30 rats were divided into five groups of six rats in each group.
Hepatoprotective and antioxidant activities of grapeseeds. Test compounds were given to rats by gavage 30 min before the ethanol administration. Amelioration of alcoholinduced hepatotoxicity by the. Jul 29, 2016 the liver performs diverse functions that are essential for life. Lcarnitine inhibits ethanolinduced gastric mucosal. Severe hepatotoxicity and nephrotoxicity of gasoline petrol. These results demonstrate that the water extract of ficus benghalensis bark is found to have significant beneficial effect on ethanol induced hepatotoxicity in wistar rats in. Preliminary toxicity study to determine the volume of gasoline that could cause toxicity was carried out using 56 healthy male albino rats. Alf was induced in male wistar rats by intraperitoneal injection of dgaln and lps. Antioxidant effect of livomap, a polyherbal formulation on. Effects of green tea extract on acute ethanolinduced. The protective actions of ethanolic extract at dose level of 200 and 400 mgkg b. The aim of the present study was to investigate the hepatoprotective activity of oleogumresin ogr in rats.
Animals were fed liquid diets and consumed approximately 60 diet per day. They were housed under standard laboratory conditions and were fed commercial rat feed lipton india ltd. Ethanol cytotoxicity to a transfected hepg2 cell line. Effect of oleogumresin on ethanol induced hepatotoxicity in rats. Ethanol and food deprivation induced enhancement of. The overall body weight of rats in formulation groups was increased while the liver weight was significantly reduced by all the formulation groups in ccl 4, ethanol, and paracetamol induced hepatotoxicity models in rats. Alcoholic hepatotoxicity is a worldwide major cause of death. These cells are a nontransformed cell line derived from normal rat liver 9. While several mechanisms likely contribute to alcohol induced liver injury, the linkage between. Ethanol etoh causes a further substantial amount of liver insufficiencies world wide. Activation of autophagy by ethanol was first examined in gfplc3 transgenic mice. Twelve male albino rats of 68 weeks old, weighing 150200 gm each were divided into two groups of 6 rats each. Accumulative data f shows the percentage of apoptosis for ndea induced hepatotoxicity in rats and pretreated with garlic and silymarin. The effect of ethanol extract mymercodia pendans on paracetamol induced hepatotoxicity in white rats article pdf available in iop conference series earth and environmental science 248.
Hepatoprotective effects of ethanol extract of caesalpiniabonduc against carbon tetrachloride induced hepatotoxicity in albino rats. Eighteen rats were randomly selected and used to evaluate the acute toxicity of the extract. Alcoholinduced retrograde memory impairment in rats. Pdf the protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Can phytochemicals be effective in preventing ethanol. Hepatoprotective potential of ethanolic extract of pandanus. After induction of liver toxicity the aqueous plant.
Male spraguedawley rats were pair fed a nutritionally adequate liquid diet containing either ethanol or isocaloric carbohydrate for 46 wk. Ethanol induced hepatotoxicity hepatoprotective study was carried out as described by. Thus, the second aim of this study was to determine whether xanthine oxidasederived oxy radicals are involved in ethanol induced, neutrophil mediated gastric mucosal injury. Different forms of rat liver aldehyde dehydrogenase and their sub cellular distribution. Administering ellagic acid to rats with ethanol induced hepatotoxicity was shown to ameliorate the increased serum levels of aminotransferases, lipid peroxides, and hydroperoxides and also to reduce the elevated hepatic contents of cholesterol, free fatty acids, triglycerides, and phospholipids. Organism names see more details, the herbal formulation showed dose dependent effect at the doses of 300 and 600 mgkg body weight and compared to 100 mgkg dose of silymarin in preventing the rise in the levels of alp, sgot, sgpt and bit. Effects of poe on oxidative damage index in alcoholic liver injury rats. Ethanol treatment to albino rats for 8 weeks induced a fair degree of derangement of liver structure and function. Protective effect of falcaria vulgaris extract on ethanol. Serum enzymes and liver histology was determined in both groups. The animals were killed 60 min after the administration of ethanol. Numerous plant resin content essential oils with terpenes and sesquiterpenes have shown hepatoprotective activity.
Induced by ethanol and carbon tetrachloride co induced hepatotoxicity in rats wenwan chao 1, shuju chen 2, huichen peng 2, jiunnwang liao 3 and sutze chou 2, 1 department of nutrition and health sciences, kainan university, taoyuan 33857, taiwan 2 department of food and nutrition, providence university, taichung 43301, taiwan. Hepatoprotective effect of guava psidium guajava l. Pharmacological evaluation of hepatoprotective activity of. Induced and chronic alcohol induced liver injury in experimental rats muhammed majeed1,2, anjali pandey2, sarang bani 2, beena bhat2, g. Group a was used as control while group b was given ethanol at a dose of 0. The liver toxicity was induced by the administration of ethanol to the animals at the optimum dosage of 7. The hepatoprotective and antioxidant roles of the gs supplementation feed against ethanol induced oxidative stress were evaluated by measuring liver damage serum marker enzymes, aspartate aminotransferase, alanine. Green tea extract ameliorates ethanol induced liver injury in albino rats poonam lodhi1, neeraj tandan2, neera singh1, divyansh kumar3 1environmental endocrinology and biomedical research unit, department of zoology, meerut college, meerut 250003, india. Methods nineweekold mice were divided into 4 groups, control, silymarin treatment, etoh treatment, and silymarinetoh treatment, with 6 mice in each group. Pdf aqueous extract of vernonia amygdalina protects. Supplementation with the hydrophobic bile salt cdc aggravates ethanol induced damage, whereas the administration of the hydrophilic bile salt udc has a protective effect.
Dec 01, 2001 the protective effects of betaine in ethanol hepatotoxicity were investigated in 24 female wistar albino rats. Effect of dietary protein and fiber on ethanolinduced. Zinc on ethanolinduced acute hepatotoxicity in adult albino rats. These results demonstrate that the water extract of ficus benghalensis bark is found to have significant beneficial effect on ethanol induced hepatotoxicity in wistar rats in reference to liver function tests performed.
The extract at doses of, 2000 and 3000 mgkg and silymarin 25 mgkg were administered for 7 days and on 7 th day all rats were challenged with ccl 4 except control group. Hepatoprotective effect of flaxseed and its protein on ethanol. Therefore the present study aims to investigate the hepatoprotective effects of salix subserrata willd flower ethanolic extract sfee against carbon tetrachloride ccl4 induced liver. Prevention of ethanol induced liver injury in rats by an agonist of peroxisome proliferatoractivated receptorgamma, pioglitazone. Antioxidant effect of hemidesmus indicus on ethanol induced hepatotoxicity in rats. Indoneesiella echioides was extracted by continuous hot percolation using ethanol.
Drug induced liver injury is a cause of acute and chronic liver disease the liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents. Because control and silymarin values were virtually identical, only control treatment is shown for ease of viewing. It was hoped that this model might prove useful in attempts to establish a cyp2e1dependent ethanol hepatotoxicity system, and to evaluate the effect of antioxidants on the ethanol toxicity, in order to establish linkage between cyp2e1, generation of reactive species and ethanol induced hepatotoxicity. Jun 30, 2014 the protective effect of fermented curcuma longa l. Amelioration of alcohol induced hepatotoxicity by the administration of ethanolic extract of sida cordifolia linn. Drug induced hepatotoxicity is the most common reason cited for withdrawal of already approved drugs from the market and accounts for more than 50 percent of cases of acute liver failure in the united states. Pdf ethanolinduced hepatotoxicity and protective effect of. Jayachitra jayaraman abstract the hypolipidemic effects of naringenin on liver fibrosis induced by exposure to ethanol in rats are investigated. Rats were divided into four groups groups 1 and 2 received isocaloric glucose and 0. Hepatoprotective activity of ethanolic extract of salix. Supplementation with the hydrophobic bile salt cdc aggravates ethanolinduced damage, whereas the administration of the hydrophilic bile salt udc has a protective effect.
Rosmarinic acid ra is an ester of caffeic acid present in many medicinal of the plant including basil, sage and. Protection of hepatotoxicity using spondias pinnata by. The pathogenesis of ald is related to the toxicity of ethanol and its. Ethanol induced lipid peroxidation male spraguedawley rats weighing 220 g were used. Using a rat in vivo model for alcoholic liver disease ald it was found that. Increased hepatotoxicity of acetaminophen after chronic. Hepatoprotective activity of ethanol extract of conyza b. Rosmarinic acid attenuates the hepatotoxicity induced by.
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